IgM Monoclonal Antibody (SA-DA4), PE, eBioscience

货号：12-9998-42

规格：100 Tests

市场价格：2271

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：SA-DA4

荧光染料：PE

类型:	人B细胞流抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5μL/test	用法：	5μL(0.06μg)/test(Flow)

产品详细信息Description:The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IgM Antibody (12-9998-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PerCP-Cy5-5 (Product # 45-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human IgM PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of neuroinflammationImmunological and clinical consequences of splenectomy in a multiple sclerosis patient treated with natalizumab."12-9998 was used in Flow cytometry/Cell sorting to analyse the peripheral blood of a splenectomised multiple sclerosis patient with natalizumab-associated progressive multifocal leukoencephalopathy."2.Frontiers in immunologySynthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells.3.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."12-9998 was used in Flow cytometry/Cell sorting to evaluate the effect of Hepatitis C virus infection on IgM+ B cell subsets."

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