IgM Monoclonal Antibody (SA-DA4), FITC, eBioscience
货号:11-9998-41,11-9998-42
规格:25 tests,100 tests
市场价格:822,1855
产品类型:流式抗体
品牌:eBioscience
抗原:IgM
物种:人
宿主:小鼠
抗体亚型:IgG1, kappa
克隆号:SA-DA4
荧光染料:FITC
类型: | 人B细胞流抗 | 同型对照: | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience |
浓度: | 5μL/test | 用法: | 5μL(0.125μg)/test(Flow) |
产品详细信息Description:The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributedto both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
IgM Antibody (11-9998-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human IgM FITC (right). Cells in the lymphocyte gate were used for analysis. |
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参考文献: |
1.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using IgM monoclonal antibody (Product # 11-9998-42) in Flow Cytometry"2.Frontiers in immunologySynthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells."Published figure using IgM monoclonal antibody (Product # 11-9998-42) in Flow Cytometry" |
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