CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience

货号：12-0088-80

规格：25 µg

市场价格：1416

产品类型：流式抗体

品牌：eBioscience

抗原：CD8

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T8

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	0.2 mg/mL	用法：	0.25 µg/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: The RPA-T8 antibody been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD8a Antibody (12-0088-80) in FlowStaining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD8a PE and CD4 PerCP-Cy5.5. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.The Journal of biological chemistryCD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers."12-0088 was used in Immunofluorescence to investigate raft localisation into CD8αβ heterodimers versus CD8αα homodimers in human cells."2.Stem cells internationalUmbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria."Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry"3.Central-European journal of immunologyCD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients."Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry"

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48796.htm