CD275 (B7-H2) Monoclonal Antibody (MIH12), APC, eBioscience

货号：17-5889-42

规格：100 Tests

市场价格：2630

产品类型：流式抗体

品牌：eBioscience

抗原：CD275

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MIH12

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MIH12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.仅用于科研。不用于诊断过程。

数据

CD275 (B7-H2) Antibody (17-5889-42) in FlowStaining of normal human peripheral blood monocyte-derived dendritic cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD275 (B7-H2) APC (purple histogram). Total viable cells, as determined by 7-AAD Viability Staining Solution (Product # 00-6993-50), were used for analysis.

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参考文献：

1. Scandinavian journal of immunologyExpression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.AuthorsYoungnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M2. Stem cell research & therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 17-5889-42) in Flow Cytometry"3. Clinical and experimental immunologyPulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 17-5889-42) in Flow Cytometry"4. OncoimmunologyTumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 17-5889-42) in Flow Cytometry"

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