CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Alexa Fluor 532, eBioscience

货号：58-0629-42,58-0629-41

规格：100 Tests,25 Tests

市场价格：3294,1406

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DREG-56 (DREG56)

荧光染料：Alexa Fluor 532

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 532, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1, and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.Applications Tested: This DREG-56 (DREG56) antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (58-0629-42) in FlowNormal human peripheral blood cells were stained with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 532 (Product # 58-4714-80) (left) or CD62L (L-Selectin) Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Stem cell research & therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 13-5889-82) in Flow Cytometry"2.Clinical and experimental immunologyPulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 13-5889-82) in Flow Cytometry"3.OncoimmunologyTumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 13-5889-82) in Flow Cytometry"4.Scandinavian journal of immunologyExpression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.AuthorsYoungnak-Piboonratanakit P,Tsushima F,Otsuki N,Igarashi H,Omura K,Azuma M5.OncoimmunologyTumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors."Published figure using CD275 (B7-H2) monoclonal antibody (Product # 13-5889-82) in Flow Cytometry"

技术参数

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