CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PerCP-eFluor 710, eBioscience

货号：46-1079-42

规格：100 tests

市场价格：3585

产品类型：流式抗体

品牌：eBioscience

抗原：CD107a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioH4A3

荧光染料：PerCP-eFluor™ 710

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test (Flow）

产品详细信息Description:The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes.LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD107a (LAMP-1) Antibody (46-1079-42) in FlowIntracellular staining of Jurkat cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD107a (LAMP-1) PerCP-eFluor® 710 (purple histogram) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.

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参考文献：

1.OncotargetPD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients."Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 46-1079-42) in Flow Cytometry"2.OncoimmunologyT cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma."Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 46-1079-42) in Flow Cytometry"3.Nature communicationsComplement receptor CD46 co-stimulates optimal human CD8+T cell effector function via fatty acid metabolism."Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 46-1079-42) in Flow Cytometry"

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