CD314 (NKG2D) Monoclonal Antibody (1D11), APC, eBioscience

货号：17-5878-42

规格：100 tests

市场价格：2788

产品类型：流式抗体

品牌：eBioscience

抗原：CD314

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D11

荧光染料：APC

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test (Flow）

产品详细信息Description:The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D11 antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (17-5878-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0086-42) and 0.5 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.5 µg of Anti-Human CD314 (NKG2D) APC (right). Total viable cells were used for analysis.

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参考文献：

1.PLoS pathogensActivation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand."17-5878 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses."2.OncoTargets and therapyPotential therapeutic strategy for gastric cancer peritoneal metastasis by NKG2D ligands-specific T cells."17-5878 was used in Flow cytometry/Cell sorting to assess expression of NKG2D ligands including MICA, MICB, and ULBP1-3 in a gastric cancer cell line and primary gastric cancer cells."

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