CD314 (NKG2D) Monoclonal Antibody (1D11), Biotin, eBioscience

货号：13-5878-82

规格：100 µg

市场价格：2580

产品类型：流式抗体

品牌：eBioscience

抗原：CD314

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D11

荧光染料：Biotin

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow）

产品详细信息Description:The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (13-5878-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0089-42) and 0.06 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (left) or 0.06 µg of Anti-Human CD314 (NKG2D) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). CD3+ cells in the lymphocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Cancer researchA chimeric receptor with NKG2D specificity enhances natural killer cell activation and killing of tumor cells."13-5878 was used in Flow cytometry/Cell sorting to show the cytotoxic potential of NK cells against tumour subtypes could be markedly enhanced by expression of NKG2D-DAP10-CD3ζ receptors."2.Bioscience reportsmiR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA."Published figure using CD314 (NKG2D) monoclonal antibody (Product # 13-5878-82) in Flow Cytometry"3.BloodNKG2D receptor regulates human effector T-cell cytokine production."13-5878 was used in Magnetic cell separation to reveal that human effector T cells alter their function depending on which innate receptors are engaged in conjunction with the TCR complex."

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48833.htm