CD314 (NKG2D) Monoclonal Antibody (1D11), PE, eBioscience

货号：12-5878-41,12-5878-42

规格：25 tests,100 tests

市场价格：1172,2663

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (12-5878-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a eFluor® 450 (Product # 48-0086-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD314 (NKG2D) PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.OncotargetPD-1 mediates functional exhaustion of activated NK cells in patients with Kaposi sarcoma."12-5878 was used in Flow cytometry/Cell sorting to propose that PD-1 mediates functional exhaustion in NK cells experiencing prolonged exposure to cells expressing activating ligands, such as tumour cells."2.PloS oneInterleukin 15 primes natural killer cells to kill via NKG2D and cPLA2 and this pathway is active in psoriatic arthritis."12-5878 was used in Flow cytometry/Cell sorting to discern the involvement of interleukin 15 in priming NK cells and discover where this pathway is active."3.Pediatric transplantationChanges in natural killer cell subsets in pediatric liver transplant recipients."12-5878 was used in Flow cytometry/Cell sorting to study levels of key immune system components before and after liver transplant."

技术参数

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