CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), eBioscience

货号：14-0629-82

规格：100 µg

市场价格：1156

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DREG-56 (DREG56)

荧光染料：其它

类型:	一抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: This DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis, non-reducing conditions-immunoblotting (WB), and immunohistology staining of frozen tissue sections. DREG-56 has also been reported in inhibition of binding to HEV. (Please use Functional Grade purified DREG-56 (DREG56), cat. 16-0629, in functional assays.).Applications Tested: The DREG-56 (DREG56) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (14-0629-82) in FlowStaining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD62P (P-Selectin) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Bioscience reportsMicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling."14-0629 was used in Western Blotting to highlight the promise of miR-140-5p as a potential target for GC treatment."AuthorsWu K,Zou J,Lin C,Jie ZG2.Nature communicationsHeterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 14-0629-82) in Flow Cytometry"3.Molecular medicine reportsn-butanol extract from Foliumisatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 14-0629-82) in Flow Cytometry"4.The Journal of clinical investigationAccelerated resolution of inflammation underlies sex differences in inflammatory responses in humans."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 14-0629-82) in Flow Cytometry"5.Journal of immunology (Baltimore, Md. : 1950)Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans."14-0629 was used in Flow cytometry/Cell sorting to investigate the link between Helios expression and natural regulatory FOXP3+ T cell subsets from different origins in humans."AuthorsHimmel ME,MacDonald KG,Garcia RV,Steiner TS,Levings MK

技术参数

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