CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Super Bright 600, eBioscience

货号：63-0629-42

规格：100 Tests

市场价格：3244

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DREG-56 (DREG56)

荧光染料：Super Bright 600

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: This DREG-56 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DREG-56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (63-0629-42) in FlowNormal human peripheral blood cells were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0048) and Mouse IgG1 kappa Isotype Control, Super Bright 600 (Product # 63-4714) (left) or CD62L Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

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技术参数

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