CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), PE-Cyanine7, eBioscience

货号：25-1038-42,25-1038-41

规格：100 Tests,25 Tests

市场价格：3090,1545

产品类型：流式抗体

品牌：eBioscience

抗原：CD103

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： B-Ly7

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.Applications Reported: This B-Ly7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B-Ly7 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteinscomposed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.仅用于科研。不用于诊断过程。

数据

CD103 (Integrin alpha E) Antibody (25-1038-42) in FlowStaining of 3-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD103 (Integrin alpha E) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells."25-1038 was used in Flow cytometry/Cell sorting to validate a novel tissue culture protocol to generate both XCR1(+) and XCR1(-) human dendritic cells from CD34(+) progenitors."AuthorsBalan S,Ollion V,Colletti N,Chelbi R,Montanana-Sanchis F,Liu H,Vu Manh TP,Sanchez C,Savoret J,Perrot I,Doffin AC,Fossum E,Bechlian D,Chabannon C,Bogen B,Asselin-Paturel C,Shaw M,Soos T,Caux C,Valladeau-Guilemond J,Dalod M2.Nature communicationsRSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."25-1038 was used in Flow cytometry/Cell sorting to study RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."3.The Journal of experimental medicineCX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22."25-1038 was used in Flow cytometry/Cell sorting to study the role of CX3CR1+ mononuclear phagocytes in the development of colitis and disease progression following Citrobacter rodentium infection."AuthorsLongman RS,Diehl GE,Victorio DA,Huh JR,Galan C,Miraldi ER,Swaminath A,Bonneau R,Scherl EJ,Littman DR

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