IgD Monoclonal Antibody (11-26c (11-26)), Alexa Fluor 700, eBioscience

货号：56-5993-82,56-5993-80

规格：100 ug,25 ug

市场价格：2445,1029

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：Alexa Fluor 700

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 700, eBioscience
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used atless than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (56-5993-82) in FlowC57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4321-80) (left) or 0.125 µg of IgD Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.

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技术参数

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