IgM Monoclonal Antibody (eB121-15F9), Biotin,

货号：13-5890-82

规格：100 µg

市场价格：1237

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB121-15F9

荧光染料：Biotin

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Biotin
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: eB121-15F9 has been reported for usein flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (13-5890-82) in FlowStaining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (left) or 0.25 µg of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献

1. PloS oneComplement-activating IgM enhances the humoral but not the T cell immune response in mice.2. GastroenterologyIndoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.3. Nature communicationsNFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

技术参数

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