CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), APC-eFluor 780, eBioscience

货号：47-0211-82

规格：100 µg

市场价格：3202

产品类型：流式抗体

品牌：eBioscience

抗原：CD21/CD35

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：eBio8D9 (8D9)

荧光染料：APC-eFluor 780

类型:	流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience
浓度：	0.2 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description:

The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is some evidence which demonstrates their expression on macrophages. CD21 is a receptor for the complement component C3d and Epstein-Barr virus (EBV). In association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2-deficient mice display impaired inflammatory and humoral immune responses in vivo.The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other, suggesting that they bind to different epitopes.Applications Reported: This 8D9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio8D9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD21/CD35 Antibody (47-0211-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Mouse CD21/CD35 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Loss of IP3Receptor-Mediated Ca2+Release in Mouse B Cells Results in Abnormal B Cell Development and Function."47-0211 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells."

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