IgD Monoclonal Antibody (11-26c (11-26)), APC, eBioscience

货号：17-5993-82,17-5993-80

规格：100 ug,25 ug

市场价格：2365,925

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：APC

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (17-5993-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.06 µg of Anti-Mouse IgD APC (right). Total viable cells were used for analysis.

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参考文献：

1.Arthritis research & therapyMast cell depletion in the preclinical phase of collagen-induced arthritis reduces clinical outcome by lowering the inflammatory cytokine profile."17-5993 was used in Flow cytometry/Cell sorting to suggest that mast cells may play a role in the regulation of the adaptive immune response during the development of arthritis."Authorsvan der Velden D,Lagraauw HM,Wezel A,Launay P,Kuiper J,Huizinga TW,Toes RE,Bot I,Stoop JN2.Cell death and differentiationMitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis."Published figure using IgD monoclonal antibody (Product # 17-5993-82) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)CD40 ligand preferentially modulates immune response and enhances protection against influenza virus."17-5993 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells."AuthorsHashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X4. Molecular and cellular biologyLoss of an Igκ gene enhancer in mature B cells results in rapid gene silencing and partial reversible dedifferentiation."17-5993 was used in Flow cytometry/Cell sorting to conclude that Igκ''s downstream enhancers are essential for the establishment and maintenance of transcriptional activity."5. Nature immunologyThe kinase TBK1 controls IgA class switching by negatively regulating noncanonical NF-κB signaling."17-5993 was used in Flow cytometry/Cell sorting to study how immunoglobulin isotype switching is controlled, showing that kinase TBK1 is a pivotal negative regulator of class switching to the immunoglobulin A (IgA) isotype, by attenuating noncanonical signaling via the transcription factor NF-κB."AuthorsJin J,Xiao Y,Chang JH,Yu J,Hu H,Starr R,Brittain GC,Chang M,Cheng X,Sun SC

技术参数

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