IgD Monoclonal Antibody (11-26c (11-26)), Biotin, eBioscience

货号：13-5993-85,13-5993-82

规格：500 ug,100 ug

市场价格：2271,1237

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：11-26c (11-26)

荧光染料：Biotin

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells.Applications Tested: The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (13-5993-82)Nature communications 2018 -Fig. 1 Loss of miR-146a in B cells led to spontaneous GC reactions in aged mice. FACS analyses and frequencies of a PNA + GL7 + GC B cells and b CXCR5 + PD-1 + Tfh cells in spleen from ~6 months old B-KO mice or their WT littermates. c Immunohistological analyses of GC reactions in SRBC-immunized spleen that were cryocut and stained with CD4 (red), PNA (green), and IgD (blue). d ELISA analysis of serum anti-dsDNA autoantibodies in aged B-KO or control mice. Data are representative of three independent experiments. Each symbol represents an individual mouse, and the bar represents the mean. Significance was determined by unpaired Student's t -test with a 95% confidence interval. ** p < 0.01, *** p < 0.001

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参考文献：

1. Cellular and molecular life sciences : CMLSDefective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice."13599382 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis"Authorsvan Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston AYear2017 2. Nature communicationsIgD attenuates the IgM-induced anergy response in transitional and mature B cells."13-5993 was used in Flow cytometry/Cell sorting to define the RNA landscape of the in vivo anergy response, comprising 220 induced sequences including a core set of 97."AuthorsSabouri Z,Perotti S,Spierings E,Humburg P,Yabas M,Bergmann H,Horikawa K,Roots C,Lambe S,Young C,Andrews TD,Field M,Enders A,Reed JH,Goodnow CCYear2016SpeciesMouseDilution1:100 3. PloS oneImmature B Cell Egress from Bone Marrow Is SOCS3 Independent."13-5993 was used in Flow cytometry/Cell sorting to investigate the role of suppressor of cytokine signaling 3 (SOCS3) in mediating immature B cell egress from the bone marrow, and other biological processes controlled by CXCR4 signalling."AuthorsNadrah K,Beck TC,Pereira JP 4. Journal of virologyUse of a virus-encoded enzymatic marker reveals that a stable fraction of memory B cells expresses latency-associated nuclear antigen throughout chronic gammaherpesvirus infection."13-5993 was used in Flow cytometry/Cell sorting to investigate the expression of LANA/EBNA-1 in individual B-cell subsets through the course of chronic gammaherpesvirus infection, showing that a stable fraction of memory B cells expresses LANA."AuthorsNealy MS,Coleman CB,Li H,Tibbetts SAYear2010SpeciesMouse 5. The Journal of experimental medicineChromosomal position of a VH gene segment determines its activation and inactivation as a substrate for V(D)J recombination."13-5993 was used in Flow cytometry/Cell sorting to study the importance of the chromosomal positioning of a VH gene segment in determining its activation and inactivation as a substrate for V(D)J recombination."AuthorsBates JG,Cado D,Nolla H,Schlissel MS

技术参数

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