IgD Monoclonal Antibody (11-26c (11-26)), PE, eBioscience

货号：12-5993-81,12-5993-82,12-5993-83

规格：50 µg,100 µg,200 µg

市场价格：1029,1855,2788

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells.Applications Tested: The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (12-5993-83) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse IgD PE (right). Total viable cells were used for analysis.

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参考文献：

1. Mucosal immunologyAntigen sampling by intestinal M cells is the principal pathway initiating mucosal IgA production to commensal enteric bacteria."12-5993 was used in Immunofluorescence to demonstrate that M-cell-mediated sampling of commensal bacteria is a required initial step for efficient induction of intestinal SIgA."AuthorsRios D,Wood MB,Li J,Chassaing B,Gewirtz AT,Williams IRYear2016SpeciesMouse 2. Journal of immunology (Baltimore, Md. : 1950)Dendritic Cells Are Dispensable for T Cell Priming and Control of Acute Lymphocytic Choriomeningitis Virus Infection."12-5993 was used in Immunohistochemistry-immunofluorescence to study the role of dendritic cells as antigen presenting cells and a source of type I interferons in acute lymphocytic choriomeningitis virus infection."AuthorsHilpert C,Sitte S,Matthies A,Voehringer D 3. PeerJDifferential effect of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) on leukocyte infiltration during contact hypersensitivity responses."12-5993 was used in Flow cytometry/Cell sorting to examine the role of PECAM-1 in the migration capacity of several different leukocyte populations after primary and secondary application."AuthorsEarly M,Schroeder WG,Unnithan R,Gilchrist JM,Muller WA,Schenkel AYear2020SpeciesMouse 4. Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."12-5993 was used in Flow cytometry/Cell sorting to support that Stat3- and BCL6-dependent extrafollicular CD4+ T and B interactions play critical functions in the pathogenesis of chronic graft-versus-host disease."AuthorsDeng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng DYear2017SpeciesMouseDilution1:300 5. OncogeneMYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis."12-5993 was used in Flow cytometry/Cell sorting to analyse the role of the anti-apoptotic protein, BCL2A1/A1, in blood cancers driven by either the MYC or ABL kinases."AuthorsSochalska M,Schuler F,Weiss JG,Prchal-Murphy M,Sexl V,Villunger A

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