IgD Monoclonal Antibody (11-26c (11-26)), eFluor 450, eBioscience

货号：48-5993-82,48-5993-80

规格：100 ug,25 ug

市场价格：2055,1029

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：11-26c (11-26)

荧光染料：eFluor 450

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (48-5993-82) in FlowStaining of C57BL/6 splenocytes withAnti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse IgD eFluor® 450 (right). Total viable cells were used for analysis.

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参考文献：

1. Frontiers in immunologyProapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner."48-5993 was used in Flow cytometry/Cell sorting to indicate that BIM regulates B cell homeostasis predominantly by limiting the life span of non-activated mature B cells."AuthorsLiu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst JYear2019SpeciesMouse 2. The Journal of experimental medicineThe transcription factor Foxo1 controls germinal center B cell proliferation in response to T cell help."48-5993 was used in Flow Cytometry to determine the role of Foxo1 in germinal center B cell proliferation."AuthorsInoue T,Shinnakasu R,Ise W,Kawai C,Egawa T,Kurosaki TYear2017SpeciesMouse 3. Nature communicationsFat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation."48-5993 was used in Flow cytometry/Cell sorting to reveal a link between inflammation, IL-33 release by FALC stromal cells, ILC2 activation and pleural B-cell activation in FALCs, resulting in local and antigen-specific IgM production."AuthorsJackson-Jones LH,Duncan SM,Magalhaes MS,Campbell SM,Maizels RM,McSorley HJ,Allen JE,Bénézech CYear2016SpeciesMouseDilution1:200 4. Arteriosclerosis, thrombosis, and vascular biologyArtery Tertiary Lymphoid Organs Control Multilayered Territorialized Atherosclerosis B-Cell Responses in Aged ApoE-/- Mice."48-5993 was used in Flow cytometry/Cell sorting to study how artery tertiary lymphoid organs can orchestrate B-cell responses in the diseased aorta."AuthorsSrikakulapu P,Hu D,Yin C,Mohanta SK,Bontha SV,Peng L,Beer M,Weber C,McNamara CA,Grassia G,Maffia P,Manz RA,Habenicht AJ5.Cell death and differentiationMitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis."Published figure using IgD monoclonal antibody (Product # 48-5993-82) in Flow Cytometry"

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