IgD Monoclonal Antibody (11-26c (11-26)), PerCP-eFluor 710, eBioscience

货号：46-5993-82,46-5993-80

规格：100 µg,25 µg

市场价格：3305,1340

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：PerCP-eFluor™ 710

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor 710, eBioscience
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (46-5993-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or Anti-Mouse IgD PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Helios Controls a Limited Subset of Regulatory T Cell Functions."46-5993 was used in Immunohistochemistry on frozen tissues to determine the beneficial effects of the transcription factor, Helios, for Treg maintenance, differentiation, and survival."AuthorsSebastian M,Lopez-Ocasio M,Metidji A,Rieder SA,Shevach EM,Thornton AM2.PloS oneBILL-cadherin/cadherin-17 contributes to the survival of memory B cells."Published figure using IgD monoclonal antibody (Product # 46-5993-82) in Flow Cytometry"3.Cell death and differentiationMitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis."Published figure using IgD monoclonal antibody (Product # 46-5993-82) in Flow Cytometry"4.Molecular and cellular biologyLoss of an Igκ gene enhancer in mature B cells results in rapid gene silencing and partial reversible dedifferentiation."Published figure using IgD monoclonal antibody (Product # 46-5993-82) in Flow Cytometry"

技术参数

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