IgD Monoclonal Antibody (11-26c (11-26)), Super Bright 600, eBioscience

货号：63-5993-82

规格：100 µg

市场价格：3226

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： 11-26c (11-26)

荧光染料：Super Bright 600

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 600, eBioscience
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used atless than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。

数据

IgD Antibody (63-5993-82) in FlowC57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4321-82) (left) or 0.25 µg of IgD Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

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技术参数

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