CD86 (B7-2) Monoclonal Antibody (GL1), PerCP-eFluor 710, eBioscience
货号:46-0862-80,46-0862-82
规格:25 μg,100 μg
市场价格:1065,2809
产品类型:流式抗体
品牌:eBioscience
抗原:CD86
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a, kappa
克隆号:GL1
荧光染料:PerCP-eFluor™ 710
类型: | 流式抗体 | 同型对照: | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor 710, eBioscience |
浓度: | 0.2 mg/mL | 用法: | 0.25 μg/test(Flow) |
产品详细信息Description:
The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
靶标信息
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
CD86 (B7-2) Antibody (46-0862-80) in FlowC57BL/6 mouse splenocytes were stimulated for 72 hours with LPS (Product # 00-4976-03) and then stained with CD19 Monoclonal Antibody, PE (Product # 12-0193-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321-82) (left) or 0.25 µg of CD86 Monoclonal Antibody, PerCP-eFluor 710 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18). |
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