CD86 (B7-2) Monoclonal Antibody (GL1), eBioscience

货号：14-0862-81,14-0862-82,14-0862-85

规格：50 μg,100 μg,500 μg

市场价格：693,1394,2564

产品类型：流式抗体

品牌：eBioscience

抗原：CD86

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：GL1

荧光染料：其它

类型:	流式抗体	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)；Assay-Dependent（IHC(F)）;Assay-Dependent(IP);Assay-Dependent(Neu)；Assay-Dependent（FN）

产品详细信息Description:

The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in blocking of CD86 in functional studies. (Please use Functional Grade purified GL1, cat. 16-0862, in functional assays.).Applications Tested: The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD86 (B7-2) Antibody (14-0862-81) in FlowStaining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Purified Anti-Mouse CD86 (B7-2) (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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参考文献：

1.Scientific reports2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex."Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0862-82) in Immunohistochemistry"2.Frontiers in cellular neuroscienceTemporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury."Published figure using CD86 (B7-2) monoclonal antibody (Product # 14-0862-82) in Flow Cytometry"3.Human molecular geneticsLoss of MyD88 alters neuroinflammatory response and attenuates early Purkinje cell loss in a spinocerebellar ataxia type 6 mouse model."14-0862 was used in Immunohistochemistry on paraffin embedded tissues to suggest that early neuroinflammatory response may play an important role in the pathogenesis of SCA6."

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