CD86 (B7-2) Monoclonal Antibody (PO3.1), Functional Grade, eBioscience

货号：16-0861-85,16-0861-38,16-0861-025,16-0861-050

规格：500 μg,5 mg,25 mg,50 mg

市场价格：3604,0,0,0

产品类型：流式抗体

品牌：eBioscience

抗原：CD86

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：PO3.1

荧光染料：其它

类型:	功能抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience
浓度：	1 mg/mL	用法：	0.25 μg/test(Flow)； Assay-Dependent（Neu）; Assay-Dependent(FN)

产品详细信息Description:

The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition toCD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The PO3.1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CD86 in functional studies.Applications Tested: The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes and can be used at 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD86 (B7-2) Antibody (16-0861-85) in FlowStaining of 2-day unstimulated (left) and 2-day LPS activated (right) C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b K Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.25 µg of Anti-Mouse CD86 (B7-2) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Total viable cells were used for analysis.

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参考文献：

1.The Journal of biological chemistryCD86 and beta2-adrenergic receptor signaling pathways, respectively, increase Oct-2 and OCA-B Expression and binding to the 3'-IgH enhancer in B cells."16-0861 was used in Western Blotting to show that CD86 stimulation up-regulates the expression of the transcription factor Oct-2 in a protein kinase C- and NF-kappaB1-dependent manner."2.Arteriosclerosis, thrombosis, and vascular biologyOral administration of an active form of vitamin D3 (calcitriol) decreases atherosclerosis in mice by inducing regulatory T cells and immature dendritic cells with tolerogenic functions."16-0861 was used in Immunohistochemistry to demonstrate that immune modulation by calcitriol may be a potentially valuable therapeutic approach against atherosclerosis."3.PloS oneAllostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype."16-0861 was used in Flow cytometry/Cell sorting to generate in culture potentially tolerogenic DC by simultaneously blocking GSK3 by lithium chloride (LiCl) and stimulating TLR2 by PAM3CysSerLys4."

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