IgM Monoclonal Antibody (II/41), APC, eBioscience 流式抗体

2024-10-28

IgM Monoclonal Antibody (II/41), APC,

货号:17-5790-82

规格:100 µg

市场价格:2892

产品类型:流式抗体

品牌:eBioscience

抗原:IgM

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:II/41

荧光染料:APC

类型:单抗同型对照:Rat IgG2a kappa Isotype Control (eBR2a), APC
浓度: 0.2 mg/mL用法:0.25 µg/test(Flow)
产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
数据

IgM Antibody (17-5790-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse IgM APC (right). Cells in the small scatter population were used for analysis.
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参考文献
1. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.2. Cell reportsModest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.3. Molecular medicine reportsAnti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.4. International journal of molecular sciencesModulation of Innate Immunity by lignin-Carbohydrate, a Novel TLR4 Ligand, Results in Augmentation of Mucosal IgA and Systemic IgG Production.5. Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

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