CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor 450, eBioscience

货号：48-0801-80,48-0801-82

规格：25 µg,100 µg

市场价格：1340,3257

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：eFluor 450

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor 450, eBioscience
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (48-0801-82) in FlowStaining of unstimulated (left) and 3-day LPS-stimulated BALB/c splenocytes (right) with 0.125 µg of Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD80 (B7-1) eFluor® 450 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 506, were used for analysis.

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参考文献：

1.Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 48-0801-82) in Flow Cytometry"2.International journal of molecular sciencescGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation FollowingMycobacterium bovisInfection."Published figure using CD80 (B7-1) monoclonal antibody (Product # 48-0801-82) in Flow Cytometry"3.Nature communicationsT cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 48-0801-82) in Flow Cytometry4.Molecular medicine reportsDiscrimination of the heterogeneity of bone marrow‑derived dendritic cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 48-0801-82) in Flow Cytometry"5.Experimental and therapeutic medicineCalreticulin is an effective immunologic adjuvant to tumor-associated antigens."Published figure using CD80 (B7-1) monoclonal antibody (Product # 48-0801-82) in Flow Cytometry"

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