CD40 Monoclonal Antibody (1C10), PE, eBioscience eBioscience 小鼠CD40单克隆抗体 PE

2024-10-28

CD40 Monoclonal Antibody (1C10), PE, eBioscience

货号:12-0401-81,12-0401-82,12-0401-83

规格:50 μg,100 μg,300 μg

市场价格:1029,2580,2892

产品类型:流式抗体

品牌:eBioscience

抗原:CD40

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:1C10

荧光染料:PE

类型: 流式抗体同型对照:Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience
浓度: 0.2 mg/mL用法:0.5 μg/test(Flow)
产品详细信息Description:

The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.The monoclonal antibody 1C10 is reported to be agonistic in vitro and in vivo.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据
CD40 Antibody (12-0401-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse CD40 PE (right). Total viable cells were used for analysis.
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参考文献:

1.CD40 Antibody (12-0401-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse CD40 PE (right). Total viable cells were used for analysis.2.Journal of immunology (Baltimore, Md. : 1950)Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression."12-0401 was used in Flow cytometry/Cell sorting to identify the functional role of collectrin in the liver."3.DiabetesProtection of islet grafts through transforming growth factor-β-induced tolerogenic dendritic cells."12-0401 was used in Flow cytometry/Cell sorting to investigate methods to achieve long-term β-islet graft tolerance for diabetes type 1, showing that islets can be protected through expression of TGF-β."

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