CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience

货号：15-0801-82,15-0801-81

规格：100 µg,50 µg

市场价格：2844,1674

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5, eBioscience
浓度：	0.2 mg/mL	用法：	0.06 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (15-0801-82) in FlowStaining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 µg of Armenian Hamster IgG Isotype Control PE-Cyanine5 (Product # 15-4888-82) (blue histogram) or 0.03 µg of Anti-Mouse CD80 (B7-1) PE-Cyanine5 (purple histogram).Total viable cells were used for analysis.

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参考文献：

1. Frontiers in immunologyNovel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research."15-0801 was used in Flow cytometry/Cell sorting to report the generation of new murine DC lines, named MutuDC, originating from cultures of splenic CD8α conventional DC (cDC) tumours."AuthorsFuertes Marraco SA,Grosjean F,Duval A,Rosa M,Lavanchy C,Ashok D,Haller S,Otten LA,Steiner QG,Descombes P,Luber CA,Meissner F,Mann M,Szeles L,Reith W,Acha-Orbea HYear2012SpeciesMouse 2. PloS oneDebridement increases survival in a mouse model of subcutaneous anthrax."15-0801 was used in Flow cytometry/Cell sorting to develop a mouse model of debridement to evaluate its effectiveness in the treatment of Bacillus anthracis soft tissue infections."AuthorsWeiner ZP,Boyer AE,Gallegos-Candela M,Cardani AN,Barr JR,Glomski IJYear2012SpeciesMouse 3. Journal of immunology (Baltimore, Md. : 1950)TLR9 and MyD88 are crucial for the development of protective immunity to malaria."15-0801 was used in Flow cytometry/Cell sorting to investigate TLR9 and MyD88 regulation of pro/anti-inflammatory cytokines in mice infected with Plasmodium yoelii."AuthorsGowda NM,Wu X,Gowda DCYear2012SpeciesMouse 4. PloS oneThe nucleosome (histone-DNA complex) is the TLR9-specific immunostimulatory component of Plasmodium falciparum that activates DCs."15-0801 was used in Flow cytometry/Cell sorting to provide a significant insight into the activation of DCs by malaria parasites."AuthorsGowda NM,Wu X,Gowda DCYear2011SpeciesMouse 5. PloS oneType I interferon drives dendritic cell apoptosis via multiple BH3-only proteins following activation by PolyIC in vivo."15-0801 was used in Flow cytometry/Cell sorting to elucidate the possible apoptotic mechanisms that control activated dendritic cell lifespan in response to PAMPs."AuthorsFuertes Marraco SA,Scott CL,Bouillet P,Ives A,Masina S,Vremec D,Jansen ES,O'Reilly LA,Schneider P,Fasel N,Shortman K,Strasser A,Acha-Orbea H

技术参数

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