IgM Monoclonal Antibody (II/41), eFluor 450,

货号：48-5790-82

规格：100 ug

市场价格：2350

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41)

荧光染料：eFluor 450

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (48-5790-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse IgM eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品

二抗荧光二抗免疫组化一抗标签抗体

参考文献

1. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.2. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.3. Cell reportsChronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.4. The FEBS journalTET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.5. Frontiers in immunologyRole of TFH Cells in Promoting T Helper 17-Induced Neuroinflammation.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48945.htm