CD80 (B7-1) Monoclonal Antibody (16-10A1), eBioscience

货号：14-0801-82,14-0801-81,14-0801-85

规格：100 µg,50 µg,500 µg

市场价格：1514,934,2684

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A

荧光染料：Purified

类型:	一抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）；Assay-Dependent（IP）；Assay-Dependent(Neu)；Assay-Dependent(FN)

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. 16-10A1 has also been reported in blocking of CD80 to its ligands. (Please use Functional Grade purified 16-10A1, cat. 16-0801, in functional assays.).Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (14-0801-82) in FlowStaining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

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参考文献：

1.Molecular medicine reportsDifferent distributions of M1 and M2 macrophages in a mouse model of laser-induced choroidal neovascularization."Published figure using CD80 (B7-1) monoclonal antibody (Product # 14-0801-82) in Immunofluorescence"2.Journal of immunology (Baltimore, Md. : 1950)B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production."14-0801 was used in Blocking experiments to show that CD28 signals are required for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets."AuthorsRibot JC,Debarros A,Mancio-Silva L,Pamplona A,Silva-Santos B3.Frontiers in microbiologyH9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375."14-0801 was used in Flow cytometry/Cell sorting to shed new light on the roles and mechanisms of PB1 and miR375 in regulating DC function and suggest new strategies for combating AIV."AuthorsLin J,Xia J,Tu CZ,Zhang KY,Zeng Y,Yang Q4.Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."Published figure using CD80 (B7-1) monoclonal antibody (Product # 14-0801-82) in Flow Cytometry"5.International journal of molecular sciencescGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation FollowingMycobacterium bovisInfection."Published figure using CD80 (B7-1) monoclonal antibody (Product # 14-0801-82) in Flow Cytometry"

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