IgM Monoclonal Antibody (II/41), PE-eFluor 610,

货号：61-5790-82

规格：100 ug

市场价格：2788

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：PE-eFluor 610

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (61-5790-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or Anti-Mouse IgM PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Genes & developmentmiR-19 is a key oncogenic component of mir-17-92.2. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.3. The FEBS journalTET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.4. Genes & developmentConversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.5. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.

技术参数

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