Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), APC, eBioscience eBioscience 小鼠NK细胞 Ly-49D单克隆抗体APC

2024-10-28

Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), APC, eBioscience

货号:17-5782-82

规格:100 µg

市场价格:2788

产品类型:流式抗体

品牌:eBioscience

抗原:Ly-49D

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:eBio4E5 (4E5)

荧光染料:APC

类型: 流式抗体同型对照:Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience
浓度: 0.2 mg/mL用法:0.5 μg/test(Flow)
产品详细信息Description:

The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R. eBio4E5 is reported to have functional (activating) activity.Applications Reported: This eBio4E5 (4E5) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio4E5 (4E5) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly-49D, an activating member of the Ly-49 family of NK cell receptors. This family of receptors form homodimers that recognize MHC Class I molecules. The activating Ly-49 receptors (Ly-49D and Ly-49H) do not contain immune receptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, distinguishing them from the inhibitory Ly-49 family members. Instead, activating Ly-49 molecules have been shown to associate with DAP12 which contains an immunoreceptor tyrosine-based activation motif (ITAM) that is required for positive signaling. Expression of different Ly-49 family members is not linked, therefore, several Ly-49 proteins can be expressed on the same cells. Expression of Ly-49D on subsets of NK cells is strain-dependent and is found in C57BL/6, CB6F1 and SJL mice, while BALB/c, DBA/2, AKR and CBA/J mice are negative for Ly-49D. In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V and Ly-49R.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据
Ly-49D Antibody (17-5782-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.25 µg of Rat IgG2a K Isotype Control APC (left) or 0.25 µg of Anti-Mouse Ly-49D APC (right). Cells in the lymphocyte gate were used for analysis.
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参考文献:

1.Journal of immunology (Baltimore, Md. : 1950)Diacylglycerol Kinase ζ Is a Target To Enhance NK Cell Function."17-5782 was used in Flow cytometry/Cell sorting to investigate methods to enhance NK cell function, showing that diacylglycerol kinase ζ is a target for enhancement of NK function."2.Nature medicineNK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors."17-5782 was used in Flow cytometry/Cell sorting to demonstrate that glioblastoma virotherapy is limited partially by an antiviral NK cell response involving specific NCRs, uncovering new potential targets to enhance cancer virotherapy."

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