货号：17-5848-80

规格：25 µg

市场价格：1237

产品类型：流式抗体

品牌：eBioscience

抗原：Ly-49E/F

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：CM4

荧光染料：APC

This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F. The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).Applications Reported: This CM4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CM4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.

The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6. Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases. Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains. Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen. Upon stimulation, expression can again be induced on Ly49E negative cells. The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).

仅用于科研。不用于诊断过程。未经明确授权不得转售。