CD335 (NKp46) Monoclonal Antibody (29A1.4), Alexa Fluor 700,

货号：56-3351-82

规格：100 µg

市场价格：2973

产品类型：流式抗体

品牌：eBioscience

抗原：CD335 (NKp46)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：29A1.4

荧光染料：Alexa Fluor 700

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 700
浓度：	0.2 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

数据

CD335 (NKp46) Antibody (56-3351-82) in FlowC57BL/6 mouse splenocytes were stained with CD49b Monoclonal Antibody, FITC (Product # 11-5971-82) and 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 700 (Product # 56-4321-80) (left) or 0.5 µg of CD335 (NKp46) Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.

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