CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade

2024-10-28

CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade

货号：16-5872-81,16-5872-82,16-5872-85,16-5872-86

规格：50μg,100μg,500μg,1mg

市场价格：1394,2103,3386,5613

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：其它

抗体类型	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade
浓度：	1 mg/mL	用法：	1 μg/test(Flow)
产品详细信息Description: The A10 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1(+) thymocytes, in vitro activated LAK cells, and a subset ofsplenic NKT cells. A10 and C7 antibodies detect NK cells from all inbred strains of mice tested so far. In redirected lysis assays, A10 is reported to stimulate NK cells to kill Daudi targets. In addition, immobilized A10 is reported to induce production of GM-CSF by NK clones and LAK cells through a CD16 (FC gamma RIII) concomitant engagement. A10 and another hamster anti-mouse NKG2D (clone C7) compete with each other for binding to transfected cells by flow cytometric analysis, suggesting that they may bind to similar epitopes or block each other by steric hindrance. C7 (neutralizing) and A10 (activating) also exhibit different functional properties.Expression of the NKG2D antigen on mouse peripheral NK and NKT cells can be detected by flow cytometric analysis using mAb CX5 with much brighter intensity.Applications Reported: The A10 antibody has been reported for use in flow cytometric analysis. It has also been reported in activation in functional studies.Applications Tested: The A10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
数据
CD314 (NKG2D) Antibody (16-5872-81) in FlowStaining of mouse splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and Anti-Mouse NKG2D PE.Total viable cells were used for analysis.
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参考文献：
1.The Journal of experimental medicineDistinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains."16-5872 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection."2.Nature communicationsRegulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis."16-5872 was used in Flow cytometry/Cell sorting to demonstrate that Chitinase-3-like-1 is a regulator of Th1 and CTL, which could be a potential anti-tumor immunity target."

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CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade

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