货号：12-5886-82

规格：100 µg

市场价格：2119

产品类型：流式抗体

品牌：eBioscience

抗原：Ly-49H

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：3D10

荧光染料：PE

The monoclonal antibody 3D10 recognizes Ly49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins. It hasbeen shown that there is preferential staining of Ly49H with Ly49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

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