Ly-49H Monoclonal Antibody (3D10), PE-Cyanine7, eBioscience eBioscience 小鼠NK细胞Ly-49H单克隆抗体PE-Cyanine7

2024-10-28

Ly-49H Monoclonal Antibody (3D10), PE-Cyanine7, eBioscience

货号:25-5886-82

规格:100 µg

市场价格:2055

产品类型:流式抗体

品牌:eBioscience

抗原:Ly-49H

物种:小鼠

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:3D10

荧光染料:PE-Cy7

类型: 流式抗体同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience
浓度: 0.2 mg/mL用法:0.5 μg/test(Flow)
产品详细信息Description:

The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. Ithas been shown that there is preferential staining of Ly-49H with Ly-49D (cat. 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D10 antibody has been tested by fow cytometric analyis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据
Ly-49H Antibody (25-5886-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) eFluor® 450 (Product # 48-5971-82) and 0.25 µg of Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or 0.25 µg of Anti-Mouse Ly-49H PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
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