Ly-49H Monoclonal Antibody (3D10), eBioscience eBioscience 小鼠NK细胞Ly-49H单克隆抗体

2024-10-28

Ly-49H Monoclonal Antibody (3D10), eBioscience

货号:14-5886-82,14-5886-37,14-5886-95

规格:100 μg,2 mg,10 mg

市场价格:1514,11562,28906

产品类型:流式抗体

品牌:eBioscience

抗原:Ly-49H

物种:小鼠

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:3D10

荧光染料:其它

类型: 流式抗体同型对照:
浓度: 0.5 mg/mL用法:0.25 μg/test(Flow);Assay-Dependent(IP);Assay-Dependent(FN)
产品详细信息Description:

The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. Ithas been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据
Ly-49H Antibody (14-5886-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971) and 0.125 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714) (left) or 0.125 µg of Anti-Mouse Ly-49H Purified (right) followed by Anti-Mouse IgG Biotin and Streptavidin APC (Product # 17-4317).Cells in the lymphocyte gate were used for analysis.

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参考文献:

1.BloodA novel Ncr1-Cre mouse reveals the essential role of STAT5 for NK-cell survival and development."14-5886 was used in Flow cytometry/Cell sorting to develop a novel mouse line to delineate the essential and cell-intrinsic role of STAT5 in NK-cell development."2.Journal of immunology (Baltimore, Md. : 1950)Genetic labeling reveals altered turnover and stability of innate lymphocytes in latent mouse cytomegalovirus infection."14-5886 was used in Flow cytometry/Cell sorting to determine that antiviral immune responses may induce sustained alterations in natural killer cell populations."3.The Journal of experimental medicineLy49H signaling through DAP10 is essential for optimal natural killer cell responses to mouse cytomegalovirus infection."14-5886 was used in Flow cytometry/Cell sorting to study Ly49H signaling through DAP10 for optimal natural killer cell responses to mouse cytomegalovirus infection."

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