Mouse anti Human CD2:Biotin 伯乐 MCA1194B CD2抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD2:Biotin 伯乐 MCA1194B CD2抗体

2024-10-28

Mouse anti Human CD2:Biotin

货号：MCA1194B

规格：0.1 mg

市场价格：2933

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD2

物种：人

宿主：小鼠

抗体亚型：IgG2b

克隆号：LT2

荧光染料：Biotin

产品介绍
Mouse anti Human CD2 antibody, clone LT2recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
Chimpanzee	√
Cynomolgus monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to biotin - liquid.
Preparation	Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin
Immunogen	Normal human peripheral blood granulocytes.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells or 100ul of whole blood using the lysed whole blood labelling method.
数据
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Blue 700 (STAR210SBB700). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.
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Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1. Moingeon, P.et al.(1989) The structural biology of CD2.Immunol Rev. 111: 111-44.2. Beyers, A.D.et al.(1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.Immunol Rev. 111: 59-77.3. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.4. Kap, Y.S.et al.(2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.J Histochem Cytochem. 57: 1159-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Human CD2:Biotin 伯乐 MCA1194B CD2抗体

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