Rat anti mouse CD19:Amethyst Orange 伯乐 MCA1439AMO CD19抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD19:Amethyst Orange 伯乐 MCA1439AMO CD19抗体

2024-10-28

Rat anti mouse CD19:Amethyst Orange

货号：MCA1439AMO

规格：0.1 mg

市场价格：4097

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD19

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号：6D5

荧光染料：Amethyst Orange

产品介绍
Rat anti Mouse CD19 antibody, clone 6D5recognizes the murine CD19 cell surface antigen, a ~95 kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Amethyst Orange - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide(NaN3) 1% Bovine Serum Albumin
Immunogen	Human K562 cell line transfected with murine CD19.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Amethyst Orange	405	540
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 1 x106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
文献
1.Starck, J.et al.(2010) Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.Blood. 116: 4795-805.2. Scotland, R.S.et al.(2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.Blood. 118 (22): 5918-27.3. White, H.N. and Meng, Q.H. (2012) Recruitment of a Distinct but Related Set of VH Sequences into the Murine CD21hi/CD23- Marginal Zone B Cell Repertoire to That Seen in the Class-Switched Antibody Response.J Immunol. 188: 287-93.4. Reynaud, J.M.et al.(2014) Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.J Virol. 88: 5421-36.5. Candolfi, M.et al.(2011) B cells are critical to T-cell-mediated antitumor immunity induced by a combined immune-stimulatory/conditionally cytotoxic therapy for glioblastoma.Neoplasia. 13: 947-60.6. Takabayashi, H.et al.(2014) Anti-inflammatory activity of bone morphogenetic protein signaling pathways in stomachs of mice.Gastroenterology. 147: 396-406.e7.7. Weiss-Gayet, M.et al.(2016) Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.PLoS One. 11 (4): e0153860.8. Meng, Q.H. & White, H.N. (2017) CD21intCD23+follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses.Immunology. 151 (2): 211-8.9. Mccubbrey, A.L.et al.(2016) MicroRNA-34a Negatively Regulates Efferocytosis by Tissue Macrophages in Part via SIRT1.J Immunol. 196 (3): 1366-75.10. Vila-Caballer, M.et al.(2019) Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice.J Mol Cell Cardiol. 132: 154-63.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti mouse CD19:Amethyst Orange 伯乐 MCA1439AMO CD19抗体

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