Mouse anti Human CD16:FITC 伯乐 MCA1193F

2024-10-28

Mouse anti Human CD16:FITC

货号:MCA1193F,MCA1193FT

规格:0.1 mg,25 µg

市场价格:2865,893

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD16

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:LNK16

荧光染料:FITC

产品介绍
Mouse anti Human CD16 antibody, clone LNK16recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).
产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

Immunogen

Normal human peripheral blood granulocytes.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Max Ex/Em490/525
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
同型

Description

Product Code

Applications

Pack Size

Mouse IgG1 Negative Control:FITC

MCA928F

F

100 Tests

相关产品

Description

Product Code

Applications

Pack Size

Human Seroblock

BUF070A

F

50 Test

Human Seroblock

BUF070B

F

200 Test

文献
1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.J Immunol. 157 (4): 1576-81.2. Fleit, H.B.et al.(1982) Human neutrophil Fc gamma receptor distribution and structure.Proc Natl Acad Sci U S A. 79 (10): 3275-9.3. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.Immunol Today. 14 (5): 215-21.4. Pillay, J.et al.(2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.J Clin Invest. 122: 327-36.5. Morris, D.L.et al.(2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.Eur J Hum Genet. 18 (9): 1027-31.6. Kamp, V.M.et al.(2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.J Leukoc Biol. 92 (5): 1011-20.7. Hanna, J.et al.(2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.Blood. 102:1569-77.8. Moalli, F.et al.(2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.Blood. 116: 5170-80.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-50301.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价