Human anti Human Ku70 antibody BIO-RAD VMA00013 Human anti Human Ku70 antibody

2024-10-28

Human anti Human Ku70 antibody

货号:VMA00013

规格:100μl

市场价格:2916

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Ku70

物种:人

宿主:其它

抗体亚型:其它

荧光染料:Purified

产品介绍
Human anti Human Ku70 antibodyrecognizes Human Ku70, also known as X-ray repair cross-complementing protein 6 or XRCC6. Ku70 is a 609 amino acid, nuclear protein involved in DNA damage repair. Ku70 contains a singleKu domainand a singleSAP domain.Ku70 forms a heterodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walkeret al.2001).Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.
产品详情
Target SpeciesHuman
Product FormA bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed inE. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
PreparationRecombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenHuman Ku70 - N1 fusion protein
AcknowledgementsPrecisionAb is a trademark of Bio-Rad Laboratories.This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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技术参数

产品应用 wb

产品类型 单克隆抗体

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