Recombinant Human Leptin BIO-RAD Recombinant Human Leptin PHP198

2024-10-29

Recombinant Human Leptin

货号:PHP198

规格:1 mg

市场价格:3162

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
Recombinant human leptinis a leptin preparation made inE. coli. Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
PreparationPurified recombinant leptin expressed inE.coli. Sterile filtered through a 0.2 micron filter
ReconstitutionReconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum abumin (BSA) is recommended.
Buffer SolutionLyophilized with 0.1% TFA
ActivityPHP198 is biologically active in theob/obmouse obesity model. Theob/obmice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Preservative StabilisersNone present
Protein Molecular Weight16.0 kDa (147 amino acid residues)
Purity>98% by SDS PAGE and HPLC
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 ELISA,WB

产品类型 重组蛋白

推荐用于 ELISA,WB

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