Mouse anti Human CD49d monoclonal antibody, clone 44H6 BIO-RAD MCA923A647T Mouse anti Human CD49d monoclonal antibody, clone

2024-10-29

Mouse anti Human CD49d monoclonal antibody, clone 44H6

货号:MCA923A647T,MCA923A647

规格:25 Tests/0.25ml,100 Tests/1ml

市场价格:1148,3213

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原: CD49d

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:44H6

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human CD49d monoclonal antibody, clone 44H6recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with sevenFG-GAPrepeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield fragments of 80 and 70 kDa (Hemleret al.1987). CD49d associates with eitherCD29to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskinet al.1997)Mouse anti human CD49d, clone 44H6 is reported to induce homotypic aggregation of pre-B cell lines includingHOONandNALM-6(Letarteet al.1993).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Alexa Fluor 647 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenHOON pre-B leukaemia cell line.
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line.
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Quackenbush, E.J. & Letarte, M. (1985) Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies.J Immunol. 134 (2): 1276-85.2.Letarte, M.et al. (1993) Homotypic aggregation of pre-B leukemic cell lines by antibodies to VLA integrins correlates with their expression of CD9.Leukemia 7(1): 93-103.3.Hartz Bet al.(2011) α 4 integrin levels on mobilized peripheral blood stem cells predict rapidity of engraftment in patients receiving autologous stem cell transplantation.Blood. 118 (8): 2362-5.4.Ling, K.L.et al.(2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.J Immunol. 178: 2908-15.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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