Mouse anti Sheep CD14 BIO-RAD MCA920GA Mouse anti Sheep CD14-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Sheep CD14 BIO-RAD MCA920GA Mouse anti Sheep CD14

2024-10-29

Mouse anti Sheep CD14

货号：MCA920GA

规格：0.1 mg

市场价格：2185

产品类型：免疫组化一抗

品牌：Bio-Rad

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

产品介绍
Mouse Anti Sheep CD14 antibody, clone VPM65recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules.
产品详情
Target Species	Sheep
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Ovine macrophages.
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Preservative Stabilisers	0.09%Sodium Azide (NaN3)
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Akesson, C.P.et al.(2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32: 837-49.2.Gupta, V.K.et al.(1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.Vet Immunol Immunopathol. 51 (1-2): 89-99.3.Summers, C.et al.(2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.Vet Immunol Immunopathol. 146: 1-7.4.Langrova, T.et al.(2008) Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharideVeterinarni Medicina, 53: 1–11

技术参数

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Mouse anti Sheep CD14 BIO-RAD MCA920GA Mouse anti Sheep CD14

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