Mouse anti Sheep CD16-Like, clone VPM64 BIO-RAD MCA919GA Mouse anti Sheep CD16-Like, clone VPM64

2024-10-29

Mouse anti Sheep CD16-Like, clone VPM64

货号:MCA919GA

规格:0.1 mg

市场价格:2185

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD16-Like

物种:绵羊

宿主:小鼠

抗体亚型:IgG1

克隆号:VPM64

荧光染料:Purified

产品介绍
Mouse anti Sheep CD16-Like, clone VPM64recognizes a cell surface antigen of 65-80kDa which is similar to the human cell surface antigen designated as CD16. It has therefore been hypothesized that clone VPM64 may recognize the ovine homologue of human CD16 (Hopkins, J.et. al.1996).Clone VPM64 recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad.Clone KD1is available purified and conjugated to FITC.
产品详情
Target SpeciesSheep
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenOvine macrophages.
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Preservative Stabilisers0.09%Sodium Azide (NaN3)
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.Vet Immunol Immunopathol. 52 (4): 329-39.2.Caroprese, M.et al.(2008) Technical note: immunomagnetic procedure for positive selection of macrophages in ovine milk.J Dairy Sci. 91:1908-12.

技术参数

产品应用 Flow;IHC

产品类型 单克隆抗体

推荐用于 Flow;IHC

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