Mouse anti Sheep TNF alpha, clone VPM61 BIO-RAD MCA905GA Mouse anti Sheep TNF alpha, clone VPM61

2024-10-29

Mouse anti Sheep TNF alpha, clone VPM61

货号:MCA905GA

规格:0.1 mg

市场价格:2185

产品类型:流式抗体

品牌:Bio-Rad

抗原:TNF alpha

物种:绵羊

宿主:小鼠

抗体亚型:IgG2a

克隆号:VPM61

荧光染料:Purified

产品介绍
Mouse anti Sheep TNF alpha, clone VPM61recognizes ovine tumor necrosis factor (TNF) alpha, a 17.5kDa cytokine expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumor and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
产品详情
Target SpeciesSheep
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenRecombinant ovine TNF alpha.
AcknowledgementsThis reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of‘The route to identification of immunological correlates of protection in ruminants’Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Preservative Stabilisers0.09%Sodium Azide (NaN3)
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Vilmos, P.et al.(1996) Phylogenetically conserved epitopes of leukocyte antigens.Vet Immunol Immunopathol. 52 (4): 415-26.2.Green, I.R.et al.(1993) Expression and characterization of bioactive recombinant ovine TNF-alpha: some species specificity in cytotoxic response to TNF.Cytokine. 5 (3): 213-23.

技术参数

产品应用 Flow;WB

产品类型 Monoclonal Antibody

推荐用于 Flow;WB

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