Mouse anti Human CD44 antibody, clone F10-44-2 BIO-RAD MCA89F Mouse anti Human CD44 antibody, clone F10-44-2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD44 antibody, clone F10-44-2 BIO-RAD MCA89F Mouse anti Human CD44 antibody, clone F10-44-2

2024-10-29

Mouse anti Human CD44 antibody, clone F10-44-2

货号：MCA89F,MCA89FT

规格：0.1 mg,25 µg

市场价格：2933,935

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD44

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：F10-44-2

荧光染料：FITC

产品介绍
Mouse anti Human CD44 antibody, clone F10-44-2recognizes human CD44, also known as Epican, HCAM, Phagocytic Glycoprotein 1 (PGP-1) or Hyaluronate receptor . Human CD44 is a single pass, type I transmembrane glycoprotein of variable molecular weight ranging from ~90kDa to ~220kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor (Laurent and Fraser 1992).CD44 isoforms containing one or more sequences encoded by the variant region exons have a much more restricted expression pattern both in terms of organ specificity and immune activation (Mackayet al.1994). .Mouse anti Human CD44 antibody, clone F10-44-2 recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackayet al.1994).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Fusion Partners	Spleen cells of immunised BALB/c mice were fused with cells from the mouse NS1 myeloma line.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Dalchau, R.et al.(1980) Monoclonal antibody to a human brain-granulocyte-T lymphocyte antigen probably homologous to the W 3/13 antigen of the rat.Eur J Immunol. 10 (10): 745-9.2.Daar, A.S. & Fabre, J.W. (1981) Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas.Lancet. 2 (8244): 434-8.3.Cattoretti, G.et al.(1993) Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections.J Pathol. 171 (2): 83-98.3.Avigdor, A.et al.(2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.Blood. 103: 2981-9.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Human CD44 antibody, clone F10-44-2 BIO-RAD MCA89F Mouse anti Human CD44 antibody, clone F10-44-2

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