Mouse anti Human CD140a antibody, clone 35248 bio-rad,Mouse anti Human CD140a antibody, clone 35248-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD140a antibody, clone 35248 bio-rad,Mouse anti Human CD140a antibody, clone 35248

2024-10-22

Mouse anti Human CD140a antibody, clone 35248

货号：0100-0220

规格：0.1 mg

价格：2576

产品类型：免疫组化一抗

品牌：Bio-Rad

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：35248 (57/20)

荧光染料：Purified

产品介绍
Mouse anti Human CD140a antibody, clone 35248recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hartet al.1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hartet al.1988). Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995).Mouse anti Human CD140a antibody, clone 35248 has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 μg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution	Borate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)5% Trehalose
Immunoge	Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.
Approx. Protein Concentrations	1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Store at -20℃ only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	0.5 µg/ml	1 µg/ml
Immunohistology - Paraffin	√	10 µg/ml	20 µg/ml
Western Blotting	√	1 µg/ml	2 µg/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
Description	Product Code	Applications	Pack Size
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5mg
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E,WB	0.5mg
文献
1De Paula, M.L.et al.(2014) The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF-1 signaling in rat and human oligodendrocyte progenitors.Glia. 62 (1): 64-77.2。Preisner, A.et al.(2015) Non-steroidal anti-inflammatory drug indometacin enhances endogenous remyelination.Acta Neuropathol. 130 (2): 247-61.3.Kuroda, M.et al.(2017) Peripherally derived FGF21 promotes remyelination in the central nervous system.J Clin Invest. 127 (9): 3496-509.

技术参数

产品应用 Elisa;WB

产品类型 Monoclonal Antibody

推荐用于 Elisa;WB

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Mouse anti Human CD140a antibody, clone 35248 bio-rad,Mouse anti Human CD140a antibody, clone 35248

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