Rat anti Human HLA DR antibody, clone YE2/36-HLK BIO-RAD MCA71F Rat anti Human HLA DR antibody, clone YE2/36-HLK

2024-10-29

Rat anti Human HLA DR antibody, clone YE2/36-HLK

货号:MCA71F

规格:0.1 mg

市场价格:3545

产品类型:流式抗体

品牌:Bio-Rad

抗原:HLA DR

物种:人

宿主:大鼠

抗体亚型:IgG2a

克隆号:YE2/36-HLK

荧光染料:FITC

产品介绍
Rat anti Human HLA DR antibody, clone YE2/36-HLKrecognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.Rat anti Human HLA DR antibody, clone YE2/36-HLK has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (Brickellet al.1981).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Fusion PartnersSpleen cells from immunised AO rats were fuzed with cells of the rat Y3 Ag1.2.3 myeloma cell line.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Davies, A.et al.(1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.J Exp Med. 170 (3): 637-54.2.Walsh, L.A.et al.(1991) Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.Eur J Immunol. 21 (3): 847-50.3.Davies, A.et al.(1995) Identification of MIC 11 antigen as an epitope of the CD59 molecule.Immunology. 85 (2): 220-7.4.Ellison BSet al.(2007) Complement susceptibility in glutamine deprived breast cancer cells.Cell Div. 2: 20.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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